Scientific Equipment from Edu-Chem Innovations

Determination of Starch Content in Foods by

UV-Vis Spectrophotometry

INTRODUCTION:

Complex Carbohydrates of high Molecular Weight, or Starches, have the unique ability to bind with Iodine (I2) and form a Deep Purple color. The intensity of the Color depends on the amount of Starch present in the test solution.

PURPOSE:

To determine the concentration (amount) of Starch in various Foods (cookies, cereals, candies, grains, etc); using KNOWN Concentrations of Starch in water solution.

INSTRUMENTS NEEDED:

EDU-6405-SPEC Scanning Spectrophotometer

(from EDU-CHEM Innovations) INFO/Buy IT!

10mm. square Cuvettes (poly-styrene or PMP / disposable-reusable) OR Glass or Quartz Cuvettes

SUPPLIES:

Text Box: FUN-SCI Kit
Includes all supplies
INFO/Buy IT! 1. Disposable plastic pipets / eyedroppers

2. Standard Starch solution 1.0%,

3. Standard Starch solution 0.50%,

4. Standard Starch solution 0.25%

5. Standard Starch solution 0.1%

6. De-Ionized Water (for "Blank" / Zero readings)

7. Iodine Test Solution [0.1N I2 / KI in boiled Water]

SAMPLES:

· Cookies,

· Cereals,

· Snack Cakes,

· Chips,

· and anything "STARCHY"

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