Measuring Caffeine in Drinks and Confections by

Liquid Chromatography

 

 

INTRODUCTION:

Caffeine is an organic molecule that can be separated from other organic molecules based on the properties of Polarity, Molecular Weight, Solubility and Molecular Geometry (steric properties).

 

 

PURPOSE:

Using the Mini-LC system optimized for the separation of Caffeine and other components in the Soda "matrix", and a simple series of Caffeine Standards in Water; we can very quickly and easily determine the amount of Caffeine in a series of popular Sodas, Teas, Coffees and Candies (Chocolate).

 

INSTRUMENTS NEEDED:

 

EDU-DLC10-HPLC w/ 254nm. UV Detector

& a mixed 65%:35% Methanol-Water Solvent Buy IT!

 

Computer operating Peak-Simple Software      Buy IT!

 

100 uL Syringe

                                                               

 

      

 

 

 SUPPLIES:

Text Box: FUN-SCI Kit Includes all supplies Buy IT!

·        Caffeine Standards at

·        100PPM,

·        250PPM

·        and 500PPM in Methanol-Water

 

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